Advances in Proteomics Research in Environmental Stress Response in Plants

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TABLE 13.1 Techniques Used in Plant Proteomic Analyzes for Subcellular Separation

Plant Species

Subcellular

Organelles

Organ

Methods

References

Hordeum vulgare

Nucleus

Root

Sorting via flow cytometry with a moderate

formaldehyde fixation

Petrovska et al.

(2014)

Nicotiana benthamiana

Ribosome

Transgenic

lines

Asymmetric flow field-flow fractionation

Pitkanen et al.

(2014)

Medicago sativa

Cell wall proteins

Stems

CaCl2, EGTA, and LiCl-complemented buffers were

used in the extraction process

Printz et al. (2015)

Solanum tuberosum

Mitochondria

Tuber

Centrifuge and layered in a Percoll density gradient

of 50%, 28%, and 20% in mannitol buffer

Salvato et al. (2014)

Arabidopsis thaliana

Leaf

Centrifugation combined with 1D-Blue native (BN)

PAGE

Senkler et al. (2017)

Arabidopsis thaliana

Plasma membrane

Seedlings

Two-phase partitioning combined with free-flow

electrophoresis

Michele et al. (2016)

Malus domestica

Chloroplasts

In vitro shoots

Sorbitol-based isolation medium with blender and

centrifugation

Morkunaite-Haimi

et al. (2018)